Detection Principles Related to
Other Immune Responses
Cell staining and appearance preservation
Scheme of phage display technology for the selection of antibody fragments.
The antibody library is amplified after transformation in E. coli and followed by
the infection of
helper phage to obtain phages displaying the antibody fragment.
phages are transferred to a surface coated with the chosen antigen. Bound
are eluted and reinfect E.coli for a new selection
round. After several rounds of the biopanning cycle, individual clones are selected
the enriched library, characterized and